Biochemistry is a scientific
field that focuses on the study of chemical compounds and processes in living
organisms. It plays a crucial role in expanding our understanding of biological
systems and how they work. Biochemistry finds applications in various fields
such as agriculture, medicine (including the pharmaceutical industry), and food
industry. Many recent developments in biology, such as genetic engineering,
biotechnology, and the molecular approach to genetic diseases, are a result of
advancements in biochemistry. Additionally, biochemistry serves as a unifying
theme in biology, as it underlies many biochemical processes such as
photosynthesis, digestion, respiration, excretion, and muscle contraction, all
of which take place within cells.
Metabolism -
Anabolism and Catabolism
Metabolism refers to the sum
of all chemical processes that occur within a cell. It consists of two
processes known as anabolism and catabolism. Anabolic reactions are those in
which simple substances are combined to form complex substances, requiring
energy. On the other hand, catabolic reactions involve the breakdown of complex
molecules into simpler ones, releasing energy.
Chemical Composition
of Cells
Cells, the basic units of life,
are composed of various biochemicals that determine their structure and
functions. Water, which constitutes around 70% of a typical mammalian cell, is
present throughout the cell and plays essential roles such as dissolving,
suspending, and ionizing materials, as well as regulating cell temperature.
Electrolytes are also present throughout the cell and help establish osmotic
gradients, pH, and membrane potential. Proteins, found in membranes,
cytoskeleton, ribosomes, and enzymes of the cell, provide structure, strength,
contractility, catalytic activity, and buffering capacity. Lipids, located in
the membranes of Golgi complex and cell inclusions, serve as reserved energy
sources, shape, protect, and insulate cells. Carbohydrates, present in the inclusions
of cells, provide fuel for metabolic activities. Nucleic acids, specifically
DNA in the nucleus, chromosome, and gene, control cell activity, while RNA in
the nucleoplasm and cytoplasm transmits genetic information and transports
amino acids. Vitamins and minerals, present as trace elements in the cytoplasm
and nucleus, work with enzymes in metabolism, maintain osmotic balance, and add
strength to buffering. In the living body, only six bioelements, out of the 16
elements and a few others that occur in particular organisms, constitute 90% of
the mass.
Relationship between
Structure and Function of Molecules
Organic molecules, which
form the building blocks of cells, consist of small molecules with carbon
chains that make up the backbone, and functional groups attached to the carbon chain
that determines their characteristics. Examples of small organic molecules in
living things include sugars, fatty acids, amino acids, and nucleosides, which
often have one or more functional groups. These small molecules can also be
units of large organic molecules, known as macromolecules or polymers, where
the small molecules are called monomers. Cells contain four classes of
macromolecules, including polysaccharides, lipids, proteins, and nucleic acids,
which play diverse roles in cells due to their variety and complexity.
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